Complement Component C3aR Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15649
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunoblotting, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunoblotting, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Complement Component C3aR 1. The exact sequence is proprietary.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 26956419).
Localization
Cell membrane
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Store as concentrated solution. Centrifuge briefly before opening vial.
Scientific Data Images for Complement Component C3aR Antibody
![Immunohistochemistry-Paraffin: Complement Component C3aR Antibody [NBP2-15649]](https://resources.bio-techne.com/images/products/Complement-Component-C3a-R-Antibody-Immunohistochemistry-Paraffin-NBP2-15649-img0007.jpg "Immunohistochemistry-Paraffin: Complement Component C3aR Antibody [NBP2-15649]")
Immunohistochemistry-Paraffin: Complement Component C3aR Antibody [NBP2-15649]
Immunohistochemistry-Paraffin: Complement Component C3aR Antibody [NBP2-15649] - Complement Component C3a R Antibody [NBP2-15649] - Tissue staining sections of mouse lung tissue without (left) and with (right) silencing by siRNA .Image submitted by a verified customer review.![Immunohistochemistry-Paraffin: Complement Component C3aR Antibody [NBP2-15649]](https://resources.bio-techne.com/images/products/Complement-Component-C3a-R-Antibody-Immunohistochemistry-Paraffin-NBP2-15649-img0003.jpg "Immunohistochemistry-Paraffin: Complement Component C3aR Antibody [NBP2-15649]")
Immunohistochemistry-Paraffin: Complement Component C3aR Antibody [NBP2-15649]
Immunohistochemistry-Paraffin: Complement Component C3a R Antibody [NBP2-15649] - Human colon carcinoma, using C3a receptor 1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Complement Component C3aR Antibody [NBP2-15649] -
Western Blot: Complement Component C3aR Antibody [NBP2-15649] - Boiled HL-60 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Complement Component C3aR antibody (NBP2-15649) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Complement Component C3aR Antibody
Application
Recommended Usage
Immunoblotting
Reported in scientific literature (PMID: 26956419).
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
Assay dependent
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Reviewed Applications
Read 1 review rated 5 using NBP2-15649 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Complement Component C3aR
Long Name
Complement Component C3a Receptor
Alternate Names
AZ3B, C3AR, C3AR1, C3R1, Complement Component C3a R
Gene Symbol
C3AR1
UniProt
Additional Complement Component C3aR Products
Product Documents for Complement Component C3aR Antibody
Product Specific Notices for Complement Component C3aR Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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