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Complement Component C5aR1 Antibody (10/92)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11241

Novus Biologicals, part of Bio-Techne
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NBP3-11241

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 10/92

Concentration

0.1 mg/ml

Product Specifications

Immunogen

RBL-2H3 transfectants expressing murine C5aR

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Complement Component C5aR1 Antibody (10/92)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Immunohistochemistry, Western-blotting and Flow Cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Complement Component C5aR1

The monoclonal antibody 10/92 recognizes the mouse receptor C5a (C5aR, CD88). The mouse anaphylatoxin C5a is a 74-residu glycopolypeptide which is generated by proteolytic cleavage of the complement factor C5 in the course of complement activation. A variety of biological effects evoked by C5a could be demonstrated, rendering this molecule an important mediator of inflammation, with granulocytes and macrophages as the main target cells. All cellular responses to C5a are specifically mediated by interactions with the membrane bound C5a receptor, a seven transmembrane GTP-binding-protein-coupled receptor that belongs to the rhodopsin supergene family. The 45 kDa C5a receptor is expressed by neutrophils, monocytes/macrophages, dendritic cells, astrocytes and microglia. In contrast, C5a receptor is not detectable on resting or stimulated murine T and B lymphocytes. The monoclonal antibody 10/92 does not inhibit the binding of C5a to its receptor.

Long Name

Complement Component C5a Receptor 1

Alternate Names

C5AR1, CD88, Complement Component C5a R1

Gene Symbol

C5AR1

Additional Complement Component C5aR1 Products

Product Documents for Complement Component C5aR1 Antibody (10/92)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Complement Component C5aR1 Antibody (10/92)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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