Complement Component C5aR1 Antibody (32-G1) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12085
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: Complement Component C5aR1 Antibody (32-G1) - Chimeric - Azide and BSA Free [NBP3-12085]](https://resources.bio-techne.com/images/products/Complement-Component-C5aR1-Antibody-32-G1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12085-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Complement Component C5aR1 Antibody (32-G1) - Chimeric - Azide and BSA Free [NBP3-12085]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 32-G1
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary
Immunogen
This antibody was raised by immunizing mice a synthetic Complement Component C5aR1 C-terminal phosphopeptide that contained phosphoserine instead of serine at position 334 and also at the neighboring phosphorylation sites Ser-332 and Ser-338.
Specificity
The 32-G1 antibody recognizes Complement Component C5aR1 which has its serine 334 phosphorylated. The mAb 32-G1 reacted specifically with a Complement Component C5aR1 peptide that contained phosphoserine 334 and also reacted with Complement Component C5aR1s that were isolated from C5a-stimulated, transfected RBL-Complement Component C5aR1 cells.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images
Immunocytochemistry/ Immunofluorescence: Complement Component C5aR1 Antibody (32-G1) - Chimeric - Azide and BSA Free [NBP3-12085]
Immunocytochemistry/Immunofluorescence: Complement Component C5aR1 Antibody (32-G1) - Chimeric [NBP3-12085] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version 32-G1 (NBP3-12085) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12085, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: Complement Component C5aR1
Long Name
Complement Component C5a Receptor 1
Alternate Names
C5AR1, CD88, Complement Component C5a R1
Gene Symbol
C5AR1
Additional Complement Component C5aR1 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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