Complement Component C5aR1 Antibody (P12/1) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-66539CL1
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG2A Clone # P12/1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
C5aR - peptide: Met1 - Asn31
Reactivity Notes
Predicted cross-reactivities: Rhesus Monkey
Localization
Multi pass membrane protein.
Specificity
Recognizes the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Clone P12/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31). Clone P12/1 does not inhibit the binding of C5a to its receptor.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for Complement Component C5aR1 Antibody (P12/1) [CoraFluor™ 1]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Complement Component C5aR1
Long Name
Complement Component C5a Receptor 1
Alternate Names
C5AR1, CD88, Complement Component C5a R1
Gene Symbol
C5AR1
Additional Complement Component C5aR1 Products
Product Documents for Complement Component C5aR1 Antibody (P12/1) [CoraFluor™ 1]
Product Specific Notices for Complement Component C5aR1 Antibody (P12/1) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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