Complement Component C5aR1 Antibody (S5/1) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12083
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: Complement Component C5aR1 Antibody (S5/1) - Chimeric - Azide and BSA Free [NBP3-12083]](https://resources.bio-techne.com/images/products/Complement-Component-C5aR1-Antibody-S5-1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12083-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Complement Component C5aR1 Antibody (S5/1) - Chimeric - Azide and BSA Free [NBP3-12083]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Bovine, Ferret, Rabbit
Applications
Block/Neutralize, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # S5/1
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary
Immunogen
This antibody was raised by immunizing mice with a synthetic peptide corresponding to C5a-R; amino acids: 1-31 (N terminal).
Specificity
S5/1 antibody recognizes the Complement Component C5aR1 (CD88) protein, a 43 kDa receptor of C5a component of the complement cascade.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images
Immunocytochemistry/ Immunofluorescence: Complement Component C5aR1 Antibody (S5/1) - Chimeric - Azide and BSA Free [NBP3-12083]
Immunocytochemistry/Immunofluorescence: Complement Component C5aR1 Antibody (S5/1) - Chimeric [NBP3-12083] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version S5/1 (NBP3-12083) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12083, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: Complement Component C5aR1
Long Name
Complement Component C5a Receptor 1
Alternate Names
C5AR1, CD88, Complement Component C5a R1
Gene Symbol
C5AR1
Additional Complement Component C5aR1 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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