Complement Factor B Antibody (M13/12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49638

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunoassay, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M13/12

Concentration

0.1 mg/ml

View all Complement Factor B Antibodies »

Product Specifications

Immunogen

The monoclonal antibody M13/12 recognizes human complement factor B/Bb.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Complement Factor B Antibody (M13/12)

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.2 um filtered solution in PBS and 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Complement Factor B

The monoclonal antibody M13/12 recognizes human complement factor B/Bb. Factor B is an acute-phase protein. Levels of factor B increase during inflammation. Complement factor B is a single- chain molecule of 764 amino acids (MW of 90 kD), including a leader peptide of 25 amino acids. Complement factor B provides the catalytic subunit of the C3/C5 convertases of the alternative complement pathway. Assembly of the C3 convertase (C3bBb) requires binding of complement factor B to C3b (C3.H2O) and factor D-mediated cleavage of bound complement factor B resulting in the release of Ba (MW 33 kD). The C3 convertase is stabilized by the binding of properdin. This provides a positive amplification loop for the classical and alternative complement pathways. Bb (MW 60 kD) is the serine protease element of this convertase. After cleavage of C3, the C5 convertase ((C3b)2Bb) is formed. The Bb fragment may be regarded as a better indicator of the alternative pathway of complement activation than Ba as impaired renal filtration does directly influence Ba levels. Whereas Bb is elevated 2.2-fold in chronic renal failure (CRF) patients, plasma concentrations of Ba are 8.4-fold higher in CRF and 16-fold higher in end-stage renal disease (ESRD) patients compared to normals. In addition to complement activation, factor B fragments participate in other immunological functions.

Alternate Names

BF, BFD, C3/C5 convertase, CFAB, CFB, GBG, H2-Bf, PBF2

Gene Symbol

CFB

Additional Complement Factor B Products

Product Documents for Complement Factor B Antibody (M13/12)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Complement Factor B Antibody (M13/12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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