Connexin 31/GJB3 Antibody (3B4-1B3)

Novus Biologicals, part of Bio-Techne | Catalog # H00002707-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B4-1B3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Connexin 31/GJB3 Antibodies »

Product Specifications

Immunogen

GJB3 (AAH12918.1, 1 a.a. ~ 270 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDWKTLQALLSGVNKYSTAFGRIWLSVVFVFRVLVYVVAAERVWGDEQKDFDCNTKQPGCTNVCYDNYFPISNIRLWALQLIFVTCPSLLVILHVAYREERERRHRQKHGDQCAKLYDNAGKKHGGLWWTYLFSLIFKLIIEFLFLYLLHTLWHGFNMPRLVQCANVAPCPNIVDCYIARPTEKKIFTYFMVGASAVCIVLTICELCYLICHRVLRGLHKDKPRGGCSPSSSASRASTCRCHHKLVEAGEVDPDPGNNKLQASAPNLTPI

Specificity

GJB3 - gap junction protein, beta 3, 31kDa (connexin 31)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Connexin 31/GJB3 Antibody (3B4-1B3)

Western Blot: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01]

Western Blot: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01]

Western Blot: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01] - Analysis of GJB3 expression in transfected 293T cell line by GJB3 monoclonal antibody (M01), clone 3B4-1B3.Lane 1: GJB3 transfected lysate(31 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01]

Immunocytochemistry/ Immunofluorescence: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01]

Immunocytochemistry/Immunofluorescence: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01] - Analysis of monoclonal antibody to GJB3 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01]

Western Blot: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01]

Western Blot: Connexin 31/GJB3 Antibody (3B4-1B3) [H00002707-M01] - GJB3 monoclonal antibody (M01), clone 3B4-1B3 Analysis of GJB3 expression in HeLa.
View 1 more images

Applications for Connexin 31/GJB3 Antibody (3B4-1B3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Connexin 31/GJB3

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene can cause non-syndromic deafness or erythrokeratodermia variabilis, a skin disorder. Alternative splicing results in multiple transcript variants encoding the same protein.

Alternate Names

DFNA2, DFNA2B, EKV, GJB3

Entrez Gene IDs

2707 (Human)

Gene Symbol

GJB3

OMIM

133200 (Human)

UniProt

AAH12918 (Human)

Additional Connexin 31/GJB3 Products

Product Documents for Connexin 31/GJB3 Antibody (3B4-1B3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Connexin 31/GJB3 Antibody (3B4-1B3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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