Connexin 32/GJB1 Antibody (GJB1/1753)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53384
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG Clone # GJB1/1753
Concentration
0.2 mg/ml
Product Summary for Connexin 32/GJB1 Antibody (GJB1/1753)
Immunogen
Recombinant human Connexin 32/GJB1 protein (Uniprot: P08034)
Localization
Cytoplasmic and Cell Surface
Specificity
This Ab recognizes a protein of 27-32kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called 'connexons' that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane therefore, one connexin usually cannot fully substitute for another.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54359)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Connexin 32/GJB1 Antibody (GJB1/1753)
Immunohistochemistry-Paraffin: Connexin 32/GJB1 Antibody (GJB1/1753) [NBP2-53384]
Immunohistochemistry-Paraffin: Connexin 32/GJB1 Antibody (GJB1/1753) [NBP2-53384] - Formalin-fixed, paraffin-embedded human Tonsil stained with Connexin 32 Monoclonal Antibody (GJB1/1753)Immunohistochemistry-Paraffin: Connexin 32/GJB1 Antibody (GJB1/1753) [NBP2-53384]
Immunohistochemistry-Paraffin: Connexin 32/GJB1 Antibody (GJB1/1753) [NBP2-53384] - Formalin-fixed, paraffin-embedded human Liver stained with Connexin 32 Monoclonal Antibody (GJB1/1753)Applications for Connexin 32/GJB1 Antibody (GJB1/1753)
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.1-0.2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Connexin 32/GJB1
Additional Connexin 32/GJB1 Products
Product Documents for Connexin 32/GJB1 Antibody (GJB1/1753)
Product Specific Notices for Connexin 32/GJB1 Antibody (GJB1/1753)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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