Connexin 36/GJD2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-59254
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Synthetic peptides corresponding to GJD2(gap junction protein, delta 2, 36kDa) The peptide sequence was selected from the middle region of GJD2. Peptide sequence ELNHLGWRKIKLAVRGAQAKRKSIYEIRNKDLPRVSVPNFGRTQSSDSAY. The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for Connexin 36/GJD2 Antibody
![Western Blot: Connexin 36/GJD2 Antibody [NBP1-59254]](https://resources.bio-techne.com/images/products/Connexin-36-GJD2-Antibody-Western-Blot-NBP1-59254-img0006.jpg "Western Blot: Connexin 36/GJD2 Antibody [NBP1-59254]")
Western Blot: Connexin 36/GJD2 Antibody [NBP1-59254]
Western Blot: Connexin 36/GJD2 Antibody [NBP1-59254] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:62500 Positive Control: Jurkat cell lysate GJD2 is supported by BioGPS gene expression data to be expressed in Jurkat
Immunohistochemistry: Connexin 36/GJD2 Antibody [NBP1-59254] -
Immunohistochemistry: Connexin 36/GJD2 Antibody [NBP1-59254] - IHC detection of Connexin 36/GJA9 in 50uM thick section of Mouse Brain. The tissues were pre-fixed overnight in 4% PFA, and primary antibody NBP1-59254 was used at a concentration of 1ug/mL (along with Parvalbumin antibody for double staining) with gentle shaking and overnight incubation at 4C. For the detection of NBP1-59254 primary, anti-rabbit Alexa 555 secondary was employed and images were captured using a confocal microscope at 63X resolution.![Western Blot: Connexin 36/GJD2 Antibody [NBP1-59254]](https://resources.bio-techne.com/images/products/Connexin-36-GJD2-Antibody-Western-Blot-NBP1-59254-img0005.jpg "Western Blot: Connexin 36/GJD2 Antibody [NBP1-59254]")
Western Blot: Connexin 36/GJD2 Antibody [NBP1-59254]
Western Blot: Connexin 36/GJD2 Antibody [NBP1-59254] - Sample Tissue: Human 293T Antibody Dilution: 1.0 ug/mlApplications for Connexin 36/GJD2 Antibody
Application
Recommended Usage
Immunohistochemistry
1:1000
Immunohistochemistry-Frozen
1:1000
Western Blot
1.0 ug/ml
Application Notes
Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 25197082). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30412665).
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Connexin 36/GJD2
Additional Connexin 36/GJD2 Products
Product Documents for Connexin 36/GJD2 Antibody
Product Specific Notices for Connexin 36/GJD2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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