Connexin 40/GJA5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46568
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
A synthetic peptide from mouse Connexin 40/GJA5 conjugated to an blue carrier protein was used as the antigen. The peptide shares 95% identity with rat sequence.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Connexin 40/GJA5 Antibody
![Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568]](https://resources.bio-techne.com/images/products/Connexin-40-GJA5-Antibody-Immunohistochemistry-Paraffin-NBP1-46568-img0003.jpg "Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568]")
Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568]
Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568] - IHC on paraffin sections of rat kidney tissue using Rabbit antibody to Connexin 40. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufactures instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer. Sections were counterstained with Harris Hematoxylin.![Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568]](https://resources.bio-techne.com/images/products/Connexin-40-GJA5-Antibody-Immunohistochemistry-Paraffin-NBP1-46568-img0001.jpg "Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568]")
Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568]
Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568] - IHC on paraffin sections of rat kidney tissue using Rabbit antibody to Connexin 40. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufactures instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer. Sections were counterstained with Harris Hematoxylin.Applications for Connexin 40/GJA5 Antibody
Application
Recommended Usage
Immunohistochemistry
1:300-1:2000
Immunohistochemistry-Paraffin
1:300-1:2000
Western Blot
1:300-1:2000
Application Notes
Western Blot. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Although not tested this antibody may work in Immunohistochemistry-Paraffin/Frozen.
Formulation, Preparation, and Storage
Purification
Unpurified
Reconstitution
Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.
Formulation
Lyophilized from whole antisera
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Connexin 40/GJA5
Additional Connexin 40/GJA5 Products
Product Documents for Connexin 40/GJA5 Antibody
Product Specific Notices for Connexin 40/GJA5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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