Connexin 43/GJA1 [p Ser368] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-722

Novus Biologicals, part of Bio-Techne
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NB100-722
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Guinea Pig (100%), Human (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Connexin 43/GJA1 Antibodies »

Product Summary for Connexin 43/GJA1 [p Ser368] Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser368 conjugated to KLH. Accession # P08050

Modification

p Ser368

Localization

Cytoplasmic and Integral membrane protein

Specificity

Specific for endogenous levels of the ~43 kDa Connexin 43/GJA1 protein phosphorylated at Ser368. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Marker

Gap Junctions Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Connexin 43/GJA1 [p Ser368] Antibody

Western Blot: Connexin 43/GJA1 [p Ser368] Antibody [NB100-722]

Western Blot: Connexin 43/GJA1 [p Ser368] Antibody [NB100-722]

Western Blot: Connexin 43/GJA1 [p Ser368] Antibody [NB100-722] - rat hippocampal lysate showing specific immunolabeling of the ~43k connexin43 phosphorylated at Ser368 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the phospho-Ser368 connexin43 antibody. The immunolabeling of connexin43 is completely eliminated by treatment.

Applications for Connexin 43/GJA1 [p Ser368] Antibody

Application
Recommended Usage

Western Blot

1:1000
Application Notes
This antibody has been tested in Western blot on rat brain hippocampal lysate, detecting a band ~43 kDa.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Connexin 43/GJA1

Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b).

Alternate Names

CX43, GJA1, GJAL, ODDD, ODOD, SDTY3

Entrez Gene IDs

2697 (Human); 14609 (Mouse); 24392 (Rat)

Gene Symbol

GJA1

UniProt

P08050 (Rat)

Additional Connexin 43/GJA1 Products

Product Documents for Connexin 43/GJA1 [p Ser368] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Connexin 43/GJA1 [p Ser368] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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