Connexin 43/GJA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-309

Novus Biologicals, part of Bio-Techne
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NB300-309
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Key Product Details

Species Reactivity

Validated:

Rat, Avian

Cited:

Avian

Predicted:

Canine (100%), Chicken (100%), Human (100%), Mouse (100%), Primate (100%), Sheep (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry (Negative), Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Connexin 43/GJA1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Accession # P08050

Reactivity Notes

It is anticipated that the antibody will also work with chicken, dog, finch, human, mouse, non-human primate, sheep, Xenopus and zebra fish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Specificity

Specific for endogenous levels of the ~43 kDa connexin43 protein.

Marker

Gap Junctions Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Connexin 43/GJA1 Antibody

Western Blot: Connexin 43/GJA1 Antibody [NB300-309]

Western Blot: Connexin 43/GJA1 Antibody [NB300-309]

Western Blot: Connexin 43/GJA1 Antibody [NB300-309] - Western blot of rat cerebellar lysate showing specific immunolabeling of the ~43k connexin43 protein.

Applications for Connexin 43/GJA1 Antibody

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Connexin 43/GJA1

Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy. Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of Connexin43 is known to be regulated by phosphorylation at Ser368 by protein kinase C. Phosphorylation of Ser368 by PKC induces a conformational change of Connexin43 that results in a decrease in gap junction permeability.

Alternate Names

CX43, GJA1, GJAL, ODDD, ODOD, SDTY3

Entrez Gene IDs

2697 (Human); 14609 (Mouse); 24392 (Rat)

Gene Symbol

GJA1

UniProt

P08050 (Rat)

Additional Connexin 43/GJA1 Products

Product Documents for Connexin 43/GJA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Connexin 43/GJA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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