Connexin 43/GJA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51938

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Predicted:

Canine (100%), Human (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Connexin 43/GJA1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QPFDFPDDNQNSKK corresponding to internal region according to NP_000156.1.

Epitope

C-QPFDFPDDNQNSKK

Marker

Gap Junctions Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Connexin 43/GJA1 Antibody

Western Blot: Connexin 43/GJA1 Antibody [NBP1-51938]

Western Blot: Connexin 43/GJA1 Antibody [NBP1-51938]

Western Blot: Connexin 43/GJA1 Antibody [NBP1-51938] - NBP1-51938 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Connexin 43/GJA1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

1:100-1:2000
Application Notes
WB: Approx. 40 kDa band observed in rat brain lysates (calculated MW of 43.0 kDa band according to human NP_000156.1 and to rat NP_036699.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Connexin 43/GJA1

The connexins are a group of GAP junction proteins, which form a hexamer to compose a connexon. Clusters of connexons form a GAP junction through which low molecular weight proteins may diffuse from cell to cell. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and GAP junction communication. There is a decrease in GAP junctional communication in Src transformed cells. The decreased communication appears to be associated with tyrosine phosphorylation of connexin 43. Activated c-Src phosphorylates the C-terminal tail of connexin 43 on residue Tyr 265, resulting in a stable interaction between both proteins leading to inhibition of GAP junctional communication. In addition to tyrosine phosphorylation, connexin 43 has also been shown to be phosphorylated on serine in the absence of Src kinases and on both serine and tyrosine in cells expressing Src kinases such as pp60v-Src and/or c-Src.

Alternate Names

CX43, GJA1, GJAL, ODDD, ODOD, SDTY3

Entrez Gene IDs

2697 (Human); 24392 (Rat)

Gene Symbol

GJA1

UniProt

NP_000156.1 (Human)

Additional Connexin 43/GJA1 Products

Product Documents for Connexin 43/GJA1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Connexin 43/GJA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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