Contactin-6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-42066

Novus Biologicals, part of Bio-Techne
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NBP3-42066
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Key Product Details

Species Reactivity

Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

0.5 mg/ml

View all Contactin-6 Antibodies »

Product Specifications

Immunogen

Immunized with recombinant Mouse Contactin-6 (Uniprot#: Q9JMB8; Tyr658-Pro907 (Uniprot#: Q9JMB8)

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for Contactin-6 Antibody

Contactin-6 Antibody

Western Blot: Contactin-6 Antibody [NBP3-42066] -

Western Blot: Contactin-6 Antibody [NBP3-42066] - Sample: Recombinant protein.
Contactin-6 Antibody

Western Blot: Contactin-6 Antibody [NBP3-42066] -

Western Blot: Contactin-6 Antibody [NBP3-42066] - Sample: Mouse Cerebrum lysate; Primary Ab: 3ug/ml NBP3-42066; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Contactin-6 Antibody

Immunohistochemistry-Paraffin: Contactin-6 Antibody [NBP3-42066] -

Immunohistochemistry-Paraffin: Contactin-6 Antibody [NBP3-42066] - Used in DAB staining on fromalin fixed paraffin- embedded Kidney tissue

Applications for Contactin-6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5-20ug/mL

Immunohistochemistry

5-20ug/mL

Western Blot

0.2-2ug/mL

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS, pH7.4, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Contactin-6

The neural adhesion molecule Contactin-6, also known as NB-3, is a contactin/F3 subgroup member of immunoglobulin superfamily. It is expressed exclusively in the nervous system and mainly upregulated at the early postnatal stage during mouse brain development. Employing Northern blot analysis Kamei et al found that amongst different regions of the adult human nervous system cerebellum expressed highest level of NB-3 mRNA. The expression of NB-3 in the cerebellum increases until adulthood. In contrast, the expression in the cerebrum declines to a low level after postnatal day 7. NB-3 like other neural recognition molecules plays a vitally important role in axonal guidance during development, plasticity, and maintenance of synaptic connections in the adult brain. Cui et al recently showed that NB-3 acts as a novel Notch ligand to participate in oligodendrocyte generation. Furthermore, NB-3 triggers nuclear translocation of the Notch intracellular domain and promotes oligodendrogliogenesis from progenitor cells and differentiation of oligodendrocyte precursor cells via Deltex1. In primary oligodendrocytes, NB-3 increases myelin-associated glycoprotein transcripts. Hence, the NB-3/Notch signaling pathway may be worthwhile a closer examination for its potential for the treatment of demyelinating diseases. Human NB-3 shares with rat NB-3 86% identity in nucleotide sequences and 90% identity in amino acid sequences. FUNCTION: Contactins mediate cell surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Involved in motor coordination. SUBCELLULAR LOCATION: Cell membrane; lipid-anchor; GPI-anchor. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Expressed in brain. In brain, it is preferentially expressed in the accessory olfactory bulb, layers II/III and V of the cerebral cortex, piriform cortex, anterior thalamic nuclei, locus coeruleus of the pons and mesencephalic trigeminal nucleus and in Purkinje cells of the cerebellum. DEVELOPMENTAL STAGE: Highly expressed after birth, reaching a maximum at the postnatal day 7, and declines thereafter in the cerebrum, whereas it increases in the cerebellum to adulthood. MISCELLANEOUS: Mice lacking CNTN6 are viable and fertile, the formation and organization of all nuclei and layers throughout the brains are apparently normal. They are however slow to learn to stay on the rotating rod in the rotorod test during repeated trials, and display dysfunction of equilibrium and vestibular senses in the wire hang and horizontal rod-walking tests. SIMILARITY: Belongs to the immunoglobulin superfamily. Contactin family. SIMILARITY: Contains 4 fibronectin type-III domains. SIMILARITY: Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

Alternate Names

CNTN6, Contactin6, NB3

Gene Symbol

CNTN6

Additional Contactin-6 Products

Product Documents for Contactin-6 Antibody

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Certificate of Analysis

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Product Specific Notices for Contactin-6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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