CoREST3/RCOR3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-60637
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 445 and 495 of human REST corepressor 3 using the numbering given in entry NP_060724.1 (GeneID 55758).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CoREST3/RCOR3 Antibody
![Western Blot: CoREST3/RCOR3 Antibody [NB100-60637]](https://resources.bio-techne.com/images/products/CoREST3-RCOR3-Antibody-Western-Blot-NB100-60637-img0002.jpg "Western Blot: CoREST3/RCOR3 Antibody [NB100-60637]")
Western Blot: CoREST3/RCOR3 Antibody [NB100-60637]
Western Blot: CoREST3/RCOR3 Antibody [NB100-60637] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NB100-60637 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RCOR3 was also immunoprecipitated by rabbit anti-RCOR3 antibody NB100-60636, which recognizes an upstream epitope.![Immunocytochemistry/ Immunofluorescence: CoREST3/RCOR3 Antibody [NB100-60637]](https://resources.bio-techne.com/images/products/CoREST3-RCOR3-Antibody-Immunocytochemistry-NB100-60637-img0004.jpg "Immunocytochemistry/ Immunofluorescence: CoREST3/RCOR3 Antibody [NB100-60637]")
Immunocytochemistry/ Immunofluorescence: CoREST3/RCOR3 Antibody [NB100-60637]
Immunocytochemistry/Immunofluorescence: CoREST3/RCOR3 Antibody [NB100-60637] - FFPE section of NBF-fixed HeLa Cells Antibody: Affinity purified rabbit anti- RCOR3 used at a dilution of 1:80 (2.5 ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight (R) 488 Conjugatedused at a dilution of 1:100.![Immunohistochemistry: CoREST3/RCOR3 Antibody [NB100-60637]](https://resources.bio-techne.com/images/products/CoREST3-RCOR3-Antibody-Immunohistochemistry-NB100-60637-img0003.jpg "Immunohistochemistry: CoREST3/RCOR3 Antibody [NB100-60637]")
Immunohistochemistry: CoREST3/RCOR3 Antibody [NB100-60637]
Immunohistochemistry: CoREST3/RCOR3 Antibody [NB100-60637] - Sample: FFPE sections of human testicular seminoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti- RCOR3 used at a dilution of 1:200 (1ug/ml) and 1:80 (2.5 ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavyApplications for CoREST3/RCOR3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 - 1:500
Immunohistochemistry
1:100-1:500
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CoREST3/RCOR3
Alternate Names
FLJ10876, FLJ16298, KIAA1343, REST corepressor 3, RP11-318L16.1
Gene Symbol
RCOR3
UniProt
Additional CoREST3/RCOR3 Products
Product Documents for CoREST3/RCOR3 Antibody
Product Specific Notices for CoREST3/RCOR3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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