CORO1B Antibody (OTI3D10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02902

Novus Biologicals, part of Bio-Techne
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NBP2-02902
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3D10

Concentration

0.64 mg/ml

View all CORO1B Antibodies »

Product Summary for CORO1B Antibody (OTI3D10)

Immunogen

Full length human recombinant protein of human CORO1B (NP_065174) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

54.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CORO1B Antibody (OTI3D10)

Western Blot: CORO1B Antibody (3D10) [NBP2-02902] Analysis of extracts (35ug) from 9 different cell lines by using anti-CORO1B monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: CORO1B Antibody (OTI3D10) [NBP2-02902]

Immunocytochemistry/ Immunofluorescence: CORO1B Antibody (OTI3D10) [NBP2-02902]

Immunocytochemistry/Immunofluorescence: CORO1B Antibody (3D10) [NBP2-02902] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CORO1B.
Flow Cytometry: CORO1B Antibody (OTI3D10) [NBP2-02902]

Flow Cytometry: CORO1B Antibody (OTI3D10) [NBP2-02902]

Flow Cytometry: CORO1B Antibody (3D10) [NBP2-02902] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CORO1B antibody, and then analyzed by flow cytometry.
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Applications for CORO1B Antibody (OTI3D10)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.64 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CORO1B

Members of the coronin family, such as CORO1B, are WD repeat-containing actin-binding proteins that regulate cell motility (Cai et al., 2005 (PubMed 16027158)).(supplied by OMIM)Regulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signaltransduction (By similarity)

Alternate Names

coronin, actin binding protein, 1B, coronin, actin-binding protein, 1B, coronin-1B, Coronin-2, DKFZp762I166

Entrez Gene IDs

57175 (Human)

Gene Symbol

CORO1B

Additional CORO1B Products

Product Documents for CORO1B Antibody (OTI3D10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CORO1B Antibody (OTI3D10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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