CORO1B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-61040

Novus Biologicals, part of Bio-Techne
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NB100-61040
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all CORO1B Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 450 and 489 of human Coronin, actin binding protein, 1B using the numbering given in entry NP_065174.1 (GeneID 57175).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CORO1B Antibody

Western Blot: CORO1B Antibody [NB100-61040]

Western Blot: CORO1B Antibody [NB100-61040]

Western Blot: CORO1B Antibody [NB100-61040] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NB100-61040 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Coronin 2 was also immunoprecipitated by rabbit anti-Coronin 2 antibody NB100-61039, which recognizes an upstream epitope.
Immunohistochemistry-Paraffin: CORO1B Antibody [NB100-61040]

Immunohistochemistry-Paraffin: CORO1B Antibody [NB100-61040]

Immunohistochemistry-Paraffin: CORO1B Antibody [NB100-61040] - Human colon carcinoma. Antibody used at a dilution of 1:1,000.
Immunohistochemistry-Paraffin: CORO1B Antibody [NB100-61040]

Immunohistochemistry-Paraffin: CORO1B Antibody [NB100-61040]

Immunohistochemistry-Paraffin: CORO1B Antibody [NB100-61040] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti-Coronin 2 used at a dilution of 1:250.

Applications for CORO1B Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:250

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CORO1B

Coronin 2 is an F-actin binding protein that plays an important role in processes involving actin dynamics such as fibroblast migration, and phagocytosis. Coronin 2 influences actin dynamics by promoting actin depolymerization. It has been shown to interact with the Arp2/3 complex and inhibit Arp2/3-induced actin nucleation. Additionally, it can regulate motility by regulating the actin severing and disassembling activity of cofilin.

Alternate Names

coronin, actin binding protein, 1B, coronin, actin-binding protein, 1B, coronin-1B, Coronin-2, DKFZp762I166

Entrez Gene IDs

57175 (Human); 23789 (Mouse)

Gene Symbol

CORO1B

UniProt

Q9BR76 (Human)

Additional CORO1B Products

Product Documents for CORO1B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CORO1B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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