CORO1B [p Ser2] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23242
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
CORO1B (Ser-2) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding serine 2 in human CORO1B. This sequence has high homology to the conserved site in rat and mouse CORO1B and in coronin-1A. However, this sequence has low homology to the conserved region in other coronins. [UniProt# Q9BR76]
Modification
p Ser2
Specificity
The antibody detects a 60 kDa band corresponding to CORO1B on SDS-PAGE immunoblots of human A431 or HeLa cells treated with Calyculin A. This band is not observed in control cells and the band observed in Calyculin A-treated cells can be removed with lambda phosphatase treatment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for CORO1B [p Ser2] Antibody
![CORO1B [p Ser2] Antibody](https://resources.bio-techne.com/images/products/nbp3-23242_rabbit-coro1b-p-ser2-pab-11920231633163.jpg "Immunocytochemistry/Immunofluorescence: CORO1B [p Ser2] Antibody [NBP3-23242] -")
Immunocytochemistry/Immunofluorescence: CORO1B [p Ser2] Antibody [NBP3-23242] -
Immunocytochemistry/Immunofluorescence: CORO1B [p Ser2] Antibody [NBP3-23242] - Immunocytochemical labeling of CORO1B in rabbit spleen fibroblasts treated with Calyculin A. The cells were labeled with rabbit polyclonal CORO1B (C-terminus) and CORO1B (Ser-2) antibodies, then detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide.![CORO1B [p Ser2] Antibody](https://resources.bio-techne.com/images/products/nbp3-23242_rabbit-coro1b-p-ser2-pab-189202317355215.jpg "Western Blot: CORO1B [p Ser2] Antibody [NBP3-23242] -")
Western Blot: CORO1B [p Ser2] Antibody [NBP3-23242] -
Western Blot: CORO1B [p Ser2] Antibody [NBP3-23242] - Western blot analysis of human A431 cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-CORO1B (C-terminal region) (lanes 1 & 2) or anti-CORO1B (Ser-2) (lanes 3 & 4).Applications for CORO1B [p Ser2] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: CORO1B
Alternate Names
coronin, actin binding protein, 1B, coronin, actin-binding protein, 1B, coronin-1B, Coronin-2, DKFZp762I166
Gene Symbol
CORO1B
Additional CORO1B Products
Product Documents for CORO1B [p Ser2] Antibody
Product Specific Notices for CORO1B [p Ser2] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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