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CORO1B [p Ser2] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23242

Novus Biologicals, part of Bio-Techne
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NBP3-23242

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for CORO1B [p Ser2] Antibody

Immunogen

CORO1B (Ser-2) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding serine 2 in human CORO1B. This sequence has high homology to the conserved site in rat and mouse CORO1B and in coronin-1A. However, this sequence has low homology to the conserved region in other coronins. [UniProt# Q9BR76]

Modification

p Ser2

Specificity

The antibody detects a 60 kDa band corresponding to CORO1B on SDS-PAGE immunoblots of human A431 or HeLa cells treated with Calyculin A. This band is not observed in control cells and the band observed in Calyculin A-treated cells can be removed with lambda phosphatase treatment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for CORO1B [p Ser2] Antibody

CORO1B [p Ser2] Antibody

Immunocytochemistry/Immunofluorescence: CORO1B [p Ser2] Antibody [NBP3-23242] -

Immunocytochemistry/Immunofluorescence: CORO1B [p Ser2] Antibody [NBP3-23242] - Immunocytochemical labeling of CORO1B in rabbit spleen fibroblasts treated with Calyculin A. The cells were labeled with rabbit polyclonal CORO1B (C-terminus) and CORO1B (Ser-2) antibodies, then detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide.
CORO1B [p Ser2] Antibody

Western Blot: CORO1B [p Ser2] Antibody [NBP3-23242] -

Western Blot: CORO1B [p Ser2] Antibody [NBP3-23242] - Western blot analysis of human A431 cells treated with Calyculin A (100 nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-CORO1B (C-terminal region) (lanes 1 & 2) or anti-CORO1B (Ser-2) (lanes 3 & 4).

Applications for CORO1B [p Ser2] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: CORO1B

Coronin 2 is an F-actin binding protein that plays an important role in processes involving actin dynamics such as fibroblast migration, and phagocytosis. Coronin 2 influences actin dynamics by promoting actin depolymerization. It has been shown to interact with the Arp2/3 complex and inhibit Arp2/3-induced actin nucleation. Additionally, it can regulate motility by regulating the actin severing and disassembling activity of cofilin.

Alternate Names

coronin, actin binding protein, 1B, coronin, actin-binding protein, 1B, coronin-1B, Coronin-2, DKFZp762I166

Gene Symbol

CORO1B

Additional CORO1B Products

Product Documents for CORO1B [p Ser2] Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CORO1B [p Ser2] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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