Cortactin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-855

Novus Biologicals, part of Bio-Techne
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NB100-855
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Bovine (100%), Canine (100%), Human (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Cortactin Antibodies »

Product Specifications

Immunogen

Peptide with sequence WKASAGHAVSIA-C corresponding to N-Terminus according to NP_005222.2, NP_612632.1.

Localization

Cytoplasmic

Specificity

This antibody is expected to recognize both reported isoforms (NP_005222.2; NP_612632.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Cortactin Antibody

Western Blot: Cortactin Antibody [NB100-855]

Western Blot: Cortactin Antibody [NB100-855]

Western Blot: Cortactin Antibody [NB100-855] - Analysis of Cortactin in Mouse Brain lysate (35ug protein in RIPA buffer) using this antibody at 0.01 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Cortactin Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 80 kDa band observed in mouse and rat brain lysates (calculated MW of 61.6 kDa band according to human NP_005222.2 and of 61.2 kDa band according to mouse NP_031829.2). The observed molecular weights correspond to earlier findings is reported in scientific literature with different antibodies (PMID 18495841).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cortactin

Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha-helical structure followed by a proline-rich region. Between the proline-rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr-421, Tyr-470, and Tyr-486). In vitro, cortactin binds to and cross-links F-actin into meshworks.

Alternate Names

Amplaxin, CTTN, EMS1

Entrez Gene IDs

2017 (Human); 13043 (Mouse)

Gene Symbol

CTTN

UniProt

NP_005222. (Human)

Additional Cortactin Products

Product Documents for Cortactin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cortactin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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