Cortactin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15970
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Predicted:
Rhesus Macaque (95%). Backed by our 100% Guarantee.
Applications
Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Cortactin Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Cortactin. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%).
Localization
Cytoplasm; cytoskeleton (By similarity); Cell projection; lamellipodium (By similarity); ruffle (By similarity)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cortactin Antibody
![Knockout Validated: Cortactin Antibody [NBP2-15970]](https://resources.bio-techne.com/images/products/Cortactin-Antibody-Western-Blot-NBP2-15970-img0004.jpg "Knockout Validated: Cortactin Antibody [NBP2-15970]")
Knockout Validated: Cortactin Antibody [NBP2-15970]
Knockout Validated: Cortactin Antibody [NBP2-15970] - Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Western Blot: Cortactin Antibody [NBP2-15970]](https://resources.bio-techne.com/images/products/Cortactin-Antibody-Western-Blot-NBP2-15970-img0001.jpg "Western Blot: Cortactin Antibody [NBP2-15970]")
Western Blot: Cortactin Antibody [NBP2-15970]
Western Blot: Cortactin Antibody [NBP2-15970] - Sample (5 ug of whole cell lysate) A: 293T B: HeLa 7. 5% SDS PAGE gel, diluted at 1:10000.Applications for Cortactin Antibody
Application
Recommended Usage
Western Blot
1:5000-1:50000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Cortactin
Additional Cortactin Products
Product Documents for Cortactin Antibody
Product Specific Notices for Cortactin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.