Cotton Rat IFN-gamma Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF565

R&D Systems, part of Bio-Techne
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BAF565
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AF565
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Key Product Details

Species Reactivity

Validated:

Cotton Rat

Cited:

Cotton Rat

Applications

Validated:

ELISA Detection (Matched Antibody Pair), Western Blot

Cited:

ELISA Development, ELISA Development (Detection), ELISpot Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all IFN-gamma Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant cotton rat IFN-gamma
His21-Ile170
Accession # Q9QXX2

Specificity

Detects cotton rat IFN-gamma in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross‑reactivity with recombinant human (rh) IFN‑ gamma, recombinant mouse (rm) IFN‑ gamma, recombinant rat (rr) IFN‑ gamma, recombinant porcine (rp) IFN‑ gamma, recombinant canine IFN‑ gamma, and recombinant feline (rfe) IFN‑ gamma is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Cotton Rat IFN-gamma Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Cotton Rat IFN-gamma (Catalog # 565-RG)

Cotton Rat IFN-gamma Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Cotton Rat IFN-gamma Antibody (Catalog # AF565)
  • Standard: Recombinant Cotton Rat IFN-gamma Protein (Catalog # 565-RG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma

Interferon-gamma (IFN-gamma), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature cotton rat IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 43%‑50% amino acid sequence identity with bovine, canine, equine, feline, human, mouse, rat, rhesus, and porcine IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

References

  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Houard, S. et al. (1999) Gene 240:107.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

Long Name

Interferon gamma

Alternate Names

IFG, IFI, IFNG, IFNgamma

Entrez Gene IDs

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Gene Symbol

IFNG

UniProt

Q9QXX2

Additional IFN-gamma Products

Product Documents for Cotton Rat IFN-gamma Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cotton Rat IFN-gamma Biotinylated Antibody

For research use only

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