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COUP-TF I/NR2F1 Antibody (JE31-95)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32211

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32211

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE31-95

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptide within Human COUP-TF I/NR2F1 aa 1-50 / 423. (Uniprot: P10589)

Localization

Nucleus

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for COUP-TF I/NR2F1 Antibody (JE31-95)

COUP-TF I/NR2F1 Antibody (JE31-95)

Western Blot: COUP-TF I/NR2F1 Antibody (JE31-95) [NBP3-32211]

Western Blot: COUP-TF I/NR2F1 Antibody (JE31-95) [NBP3-32211] - Western blot analysis of COUP-TF I/NR2F1 on different lysates with Rabbit anti-COUP-TF I/NR2F1 antibody (NBP3-32211) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (10 ug/Lane)
Lane 2: Human brain tissue lysate (40 ug/Lane)

Predicted band size: 46 kDa
Observed band size: 46 kDa

Exposure time: 8 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32211) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
COUP-TF I/NR2F1 Antibody (JE31-95)

Immunohistochemistry: COUP-TF I/NR2F1 Antibody (JE31-95) [NBP3-32211]

Immunohistochemistry: COUP-TF I/NR2F1 Antibody (JE31-95) [NBP3-32211] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-COUP-TF I/NR2F1 antibody (NBP3-32211) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32211) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for COUP-TF I/NR2F1 Antibody (JE31-95)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: COUP-TF I/NR2F1

COUP TF1, or Coup (chicken ovalbumin upstream promoter) transcription factor, binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. It has varied roles in the development of the peripheral nervous system, such as early regionalization of the neocortex, differentiation of subplate neurons and guidance of thalamocortical axons.

Long Name

COUP Transcription Factor 1/Nuclear Receptor Subfamily 2, Group F, Member 1

Alternate Names

COUPTF I, ERBAL3, NR2F1

Gene Symbol

NR2F1

Additional COUP-TF I/NR2F1 Products

Product Documents for COUP-TF I/NR2F1 Antibody (JE31-95)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for COUP-TF I/NR2F1 Antibody (JE31-95)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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