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COUP-TF I/NR2F1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15974

Novus Biologicals, part of Bio-Techne
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NBP2-15974

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rat (99%), Xenopus (98%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for COUP-TF I/NR2F1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human COUP-TF I/NR2F1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (98%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for COUP-TF I/NR2F1 Antibody

Western Blot: COUP-TF I/NR2F1 Antibody [NBP2-15974]

Western Blot: COUP-TF I/NR2F1 Antibody [NBP2-15974]

Western Blot: COUP-TF I/NR2F1 Antibody [NBP2-15974] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with COUP TF1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: COUP-TF I/NR2F1 Antibody [NBP2-15974]

Western Blot: COUP-TF I/NR2F1 Antibody [NBP2-15974]

Western Blot: COUP-TF I/NR2F1 Antibody [NBP2-15974] - Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE gel, diluted at 1:1000.

Applications for COUP-TF I/NR2F1 Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000
Application Notes
Immunoprecipitation Assay-dependent dilution
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: COUP-TF I/NR2F1

Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.

Long Name

COUP Transcription Factor 1/Nuclear Receptor Subfamily 2, Group F, Member 1

Alternate Names

COUPTF I, ERBAL3, NR2F1

Gene Symbol

NR2F1

UniProt

Additional COUP-TF I/NR2F1 Products

Product Documents for COUP-TF I/NR2F1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for COUP-TF I/NR2F1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.