COX-2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB110-1948
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit Serum
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a fusion protein corresponding to the carboxy-terminus of human COX-2. (Uniprot: P35354)
Reactivity Notes
Reactivity against homologues from other sources is not known
Localization
Membrane associated. Microsomal membrane.
Specificity
This antiserum is directed against human COX-2. Two different isoforms of the enzyme are known, Cox-1 and COX-2. A BLAST analysis was used to suggest cross-reactivity with COX-2 in human, mouse and rat based on a homology with the immunizing sequence and should not cross react with Cox-1. Reactivity against homologues from other sources is not known. Reactivity of this antibody with COX-2 from other species is unknown
Clonality
Polyclonal
Host
Rabbit
Isotype
Serum
Description
This antiserum is directed against human Cox-2
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for COX-2 Antibody
![Western Blot: COX-2 Antibody [NB110-1948]](https://resources.bio-techne.com/images/products/COX-2-Antibody-Western-Blot-NB110-1948-img0001.jpg "Western Blot: COX-2 Antibody [NB110-1948]")
Western Blot: COX-2 Antibody [NB110-1948]
Western Blot: COX-2 Antibody [NB110-1948] - COX-2 antibody is shown to detect COX-2 present in COX-2 transfected Sf9 cell extract (lane 1) and IL-1beta induced WISH cell extract (lane 2). Detection occurs using a 1:10,000 dilution of antibody followed by 1:10,000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1'. Other detection systems will yield similar results.
COX-2 Antibody
anti-Cox2 is shown to detect Cox-2 present in Cox-2 transfected Sf9 cell extract (lane 1) and IL-1beta induced WISH cell extract (lane 2). Detection occurs using a 1:10,000 dilution of antibody followed by 1:10,000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1'. Other detection systems will yield similar results.Applications for COX-2 Antibody
Application
Recommended Usage
ELISA
1:10000-1:50000
Immunohistochemistry
1:10-1:500
Western Blot
1:5000-1:10000
Application Notes
Cox-2 (Cyclooxygenase-2) is an inducible enzyme that is normally absent from cells, however, in response to growth factors, tumor promoters and some cytokines, it undergoes a rapid and transient expression. This antiserum against Human Cox-2 and has been tested for use in immunoblotting. The antibody recognizes Cox-2 at 70kDa in Sf9 cell lines transfected with Cox-2 as well as in WISH cells induced with IL-1b. Reactivity in other immunoassays is unknown.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Antiserum
Preservative
0.01% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: COX-2
Long Name
Cyclooxygenase 2
Alternate Names
COX2, PGHS-2, PHS-II, PTGS2
Gene Symbol
PTGS2
UniProt
Additional COX-2 Products
Product Documents for COX-2 Antibody
Product Specific Notices for COX-2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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