COX4 Antibody (854)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43540

Novus Biologicals, part of Bio-Techne
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NBP2-43540
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Predicted:

Chimpanzee (100%), Rhesus Macaque (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 854

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all COX4 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.

Reactivity Notes

Bovine (81%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for COX4 Antibody (854)

Western Blot: COX4 Antibody (854) [NBP2-43540]

Western Blot: COX4 Antibody (854) [NBP2-43540]

Western Blot: COX4 Antibody (854) [NBP2-43540] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (854)diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: COX4 Antibody (854) [NBP2-43540]

Immunohistochemistry-Paraffin: COX4 Antibody (854) [NBP2-43540]

Immunohistochemistry-Paraffin: COX4 Antibody (854) [NBP2-43540] - Human colon cancer. COX4 stained by COX4 antibody [854] diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
Western Blot: COX4 Antibody (854) [NBP2-43540]

Western Blot: COX4 Antibody (854) [NBP2-43540]

Western Blot: COX4 Antibody (854) [NBP2-43540] - Analysis of A. 50 ug mouse heart lysate/extract 12 % SDS-PAGE COX4 antibody dilution: 1:1000
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Applications for COX4 Antibody (854)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Simple Western

reported by internal validation

Western Blot

1:500-1:3000
Application Notes
In Simple Western internal validation: Antibody dilution of 6 ug/ml; matrix was 12-230 kDa; detected by Chemiluminescence.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: COX4

COX IV (cytochrome c oxidase subunit IV isoform 1) plays a pivotal role in respiratory function and assembly of the enzyme complex. This protein is known to have interactions with MT-CO2, MCL1, TMBIM4, MT-CO1 and TH1L. COX IV has been studied in relation to anemia, Huntington's disease, Alzheimer's disease, neuronitis, pancreatitis and several other diseases and disorders.

Long Name

Cytochrome c Oxidase Subunit IV

Alternate Names

COX4I2

Gene Symbol

COX4I1

Additional COX4 Products

Product Documents for COX4 Antibody (854)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for COX4 Antibody (854)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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