COX4 Antibody (CL3501)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59777

Novus Biologicals, part of Bio-Techne
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NBP2-59777
NBP2-59777-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL3501

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all COX4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LATRVFSLVGKRAISTSVCVRAHESVVKSEDFSLPAYMDRRDHPLPEVAHVKHLSASQKALKEKEKASWSSLSMDEKVELYRIKFKESFAEMNRGSNEWKT

Epitope

EKVELYRIKFKESFA

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for COX4 Antibody (CL3501)

Western Blot: COX4 Antibody (CL3501) [NBP2-59777]

Western Blot: COX4 Antibody (CL3501) [NBP2-59777]

Western Blot: COX4 Antibody (1327) [NBP2-59777] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-COX4I1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.
Immunohistochemistry-Paraffin: COX4 Antibody (CL3501) [NBP2-59777]

Immunohistochemistry-Paraffin: COX4 Antibody (CL3501) [NBP2-59777]

Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human liver shows cytoplasmic immunoreactivity in hepatocytes.
Western Blot: COX4 Antibody (CL3501) [NBP2-59777]

Western Blot: COX4 Antibody (CL3501) [NBP2-59777]

Western Blot: COX4 Antibody (1327) [NBP2-59777] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2 and mouse cell line NIH-3T3.
View 4 more images

Applications for COX4 Antibody (CL3501)

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: COX4

COX IV (cytochrome c oxidase subunit IV isoform 1) plays a pivotal role in respiratory function and assembly of the enzyme complex. This protein is known to have interactions with MT-CO2, MCL1, TMBIM4, MT-CO1 and TH1L. COX IV has been studied in relation to anemia, Huntington's disease, Alzheimer's disease, neuronitis, pancreatitis and several other diseases and disorders.

Long Name

Cytochrome c Oxidase Subunit IV

Alternate Names

COX4I2

Gene Symbol

COX4I1

Additional COX4 Products

Product Documents for COX4 Antibody (CL3501)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for COX4 Antibody (CL3501)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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