COX5b Antibody (020)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90311
Recombinant Monoclonal Antibody
![Western Blot: COX5b Antibody (020) [NBP2-90311]](https://resources.bio-techne.com/images/products/COX5b-Antibody-020-Western-Blot-NBP2-90311-img0001.jpg "Western Blot: COX5b Antibody (020) [NBP2-90311]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 020
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human COX5b (Uniprot#: P10606; Ala32-His129).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for COX5b Antibody (020)
Western Blot: COX5b Antibody (020) [NBP2-90311]
Western Blot: COX5b Antibody (020) [NBP2-90311] - Lane A: HepG2 Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:14 kDaObserved band size:15 kDa![Immunoprecipitation: COX5b Antibody (020) [NBP2-90311]](https://resources.bio-techne.com/images/products/COX5b-Antibody-020-Immunoprecipitation-NBP2-90311-img0002.jpg "Immunoprecipitation: COX5b Antibody (020) [NBP2-90311]")
Immunoprecipitation: COX5b Antibody (020) [NBP2-90311]
Immunoprecipitation: COX5b Antibody (020) [NBP2-90311] - Lane A:0.5 mg A549 Whole Cell Lysate. Lane B:0.5 mg Hela Whole Cell Lysate. Lane C:0.5 mg HepG2 Whole Cell Lysate. 2 uL anti-COX5B rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-COX5B rabbit monoclonal antibody,at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique.Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 14 kDaApplications for COX5b Antibody (020)
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: COX5b
Alternate Names
COXVB, Cytochrome c oxidase polypeptide Vb, cytochrome c oxidase polypeptide VB, mitochondrial, cytochrome c oxidase subunit 5B, mitochondrial, cytochrome c oxidase subunit Vb
Gene Symbol
COX5B
Additional COX5b Products
Product Documents for COX5b Antibody (020)
Product Specific Notices for COX5b Antibody (020)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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