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COX5b Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31303

Novus Biologicals, part of Bio-Techne
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NBP1-31303

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX5B. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Canine (83%), Porcine (83%), Rat (82%), Bovine (84%).

Localization

Mitochondrion inner membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for COX5b Antibody

Immunohistochemistry-Paraffin: COX5b Antibody [NBP1-31303]

Immunohistochemistry-Paraffin: COX5b Antibody [NBP1-31303]

Immunohistochemistry-Paraffin: COX5b Antibody [NBP1-31303] - Paraffin-embedded H441 xenograft, using antibody at 1:100 dilution.
COX5b Antibody

Western Blot: COX5b Antibody [NBP1-31303] -

Western Blot: COX5b Antibody [NBP1-31303] - Sample(30 ug of whole cell lysate)
A:Hep G2
15% SDS PAGE
NBP1-31303 diluted at 1:1000

Applications for COX5b Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: COX5b

Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme.

Alternate Names

COXVB, Cytochrome c oxidase polypeptide Vb, cytochrome c oxidase polypeptide VB, mitochondrial, cytochrome c oxidase subunit 5B, mitochondrial, cytochrome c oxidase subunit Vb

Gene Symbol

COX5B

Additional COX5b Products

Product Documents for COX5b Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for COX5b Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.