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CPNE1 Antibody (8B8)

Novus Biologicals, part of Bio-Techne | Catalog # H00008904-M01

Novus Biologicals, part of Bio-Techne
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H00008904-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 8B8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

CPNE1 (NP_003906, 111 a.a. ~ 210 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TLPLMLKPGKPAGRGTITVSAQELKDNRVVTMEVEARNLDKKDFLGKSDPFLEFFRQGDGKWHLVYRSEVIKNNLNPTWKRFSVPVQHFCGGNPSTPIQV

Specificity

CPNE1 - copine I

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CPNE1 Antibody (8B8)

Western Blot: CPNE1 Antibody (8B8) [H00008904-M01]

Western Blot: CPNE1 Antibody (8B8) [H00008904-M01]

Western Blot: CPNE1 Antibody (8B8) [H00008904-M01] - CPNE1 monoclonal antibody (M01), clone 8B8 Analysis of CPNE1 expression in HeLa.
Western Blot: CPNE1 Antibody (8B8) [H00008904-M01]

Western Blot: CPNE1 Antibody (8B8) [H00008904-M01]

Western Blot: CPNE1 Antibody (8B8) [H00008904-M01] - Analysis of CPNE1 over-expressed 293 cell line, cotransfected with CPNE1 Validated Chimera RNAi ( Cat # H00008904-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CPNE1 monoclonal antibody (M01), clone 8B8 (Cat # H00008904-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: CPNE1 Antibody (8B8) [H00008904-M01]

Western Blot: CPNE1 Antibody (8B8) [H00008904-M01]

Western Blot: CPNE1 Antibody (8B8) [H00008904-M01] - Analysis of CPNE1 expression in transfected 293T cell line by CPNE1 monoclonal antibody (M01), clone 8B8.Lane 1: CPNE1 transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.

Applications for CPNE1 Antibody (8B8)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CPNE1

Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene encodes a calcium-dependent protein that also contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. However, the encoded protein does not contain a predicted signal sequence or transmembrane domains. This protein has a broad tissue distribution and it may function in membrane trafficking. This gene and the gene for RNA binding motif protein 12 overlap at map location 20q11.21. Sequence analysis identified multiple alternatively spliced variants in the 5' UTR. All variants encode the same protein.

Alternate Names

copine ICPN1COPN1, copine-1, MGC1142

Entrez Gene IDs

8904 (Human)

Gene Symbol

CPNE1

OMIM

604205 (Human)

Additional CPNE1 Products

Product Documents for CPNE1 Antibody (8B8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CPNE1 Antibody (8B8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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