CPSF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-79822

Novus Biologicals, part of Bio-Techne
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NB100-79822
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all CPSF1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1392 and 1442 of human cleavage and polyadenylation specific factor 1, 160kD subunit using the numbering given in entry NP_037423.1 (GeneID 29894).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CPSF1 Antibody

Western Blot: CPSF1 Antibody [NB100-79822]

Western Blot: CPSF1 Antibody [NB100-79822]

Western Blot: CPSF1 Antibody [NB100-79822] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-CPSF160 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescencewith an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822]

Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822]

Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CPSF160 (NB100-79822). Detection: DAB
Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822]

Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822]

Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-CPSF160 (NB100-79822). Detection: DAB
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Applications for CPSF1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CPSF1

Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis. CPSF1 is the largest subunit of the CPSF complex (Murthy and Manley, 1995 [PubMed 7590244]).[supplied by OMIM]

Alternate Names

cleavage and polyadenylation specific factor 1, 160kD subunit, cleavage and polyadenylation specific factor 1, 160kDa, Cleavage and polyadenylation specificity factor 160 kDa subunit, cleavage and polyadenylation specificity factor subunit 1, CPSF 160 kDa subunit, CPSF160, HSU37012, P/cl.18, polyadenylation specificity factor

Entrez Gene IDs

29894 (Human); 94230 (Mouse)

Gene Symbol

CPSF1

UniProt

Q10570 (Human)

Additional CPSF1 Products

Product Documents for CPSF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CPSF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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