CPT1A Antibody (3M6E9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16072

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3M6E9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CPT1A Antibodies »

Product Summary for CPT1A Antibody (3M6E9)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 520-720 of human CPT1A (NP_001867.2). WDIPGECQEVIETSLNTANLLANDVDFHSFPFVAFGKGIIKKCRTSPDAFVQLALQLAHYKDMGKFCLTYEASMTRLFREGRTETVRSCTTESCDFVRAMVDPAQTVEQRLKLFKLASEKHQHMYRLAMTGSGIDRHLFCLYVVSKYLAVESPFLKEVLSEPWRLSTSQTPQQQVELFDLENNPEYVSSGGGFGPVADDGY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CPT1A Antibody (3M6E9)

Western Blot: CPT1A Antibody (3M6E9) [NBP3-16072]

Western Blot: CPT1A Antibody (3M6E9) [NBP3-16072]

Western Blot: CPT1A Antibody (3M6E9) [NBP3-16072] - Analysis of Mouse heart, using CPT1A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.
Western Blot: CPT1A Antibody (3M6E9) [NBP3-16072]

Western Blot: CPT1A Antibody (3M6E9) [NBP3-16072]

Western Blot: CPT1A Antibody (3M6E9) [NBP3-16072] - Analysis of various lysates, using CPT1A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.

Applications for CPT1A Antibody (3M6E9)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CPT1A

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation.

Long Name

Carnitine O-palmitoyltransferase 1, liver isoform

Alternate Names

CPT I, CPT1, CPT1-L, CPTI-L, EC 2.3.1.21

Gene Symbol

CPT1A

Additional CPT1A Products

Product Documents for CPT1A Antibody (3M6E9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CPT1A Antibody (3M6E9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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