CPT1A Antibody (HL1164) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25423
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Canine, Feline
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1164
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for CPT1A Antibody (HL1164) - Azide and BSA Free
Immunogen
Recombinant protein encompassing a sequence within the center region of human CPT1A. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (82%), Sheep (85%), Bovine (86%), Porcine (89%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CPT1A Antibody (HL1164) - Azide and BSA Free
Knockout Validated: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] -
Knockout Validated: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - Wild-type (WT) and CPT1A knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Paraffin: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] -
Immunohistochemistry-Paraffin: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - CPT1A antibody [HL1164] detects CPT1A protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded dog intestine. CPT1A stained by CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] -
Western Blot: CPT1A Antibody (HL1164) - Azide and BSA Free [NBP3-25423] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (NBP3-25423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Applications for CPT1A Antibody (HL1164) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CPT1A
Long Name
Carnitine O-palmitoyltransferase 1, liver isoform
Alternate Names
CPT I, CPT1, CPT1-L, CPTI-L, EC 2.3.1.21
Gene Symbol
CPT1A
Additional CPT1A Products
Product Documents for CPT1A Antibody (HL1164) - Azide and BSA Free
Product Specific Notices for CPT1A Antibody (HL1164) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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