CPT1A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15993

Novus Biologicals, part of Bio-Techne
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NBP2-15993
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CPT1A Antibodies »

Product Summary for CPT1A Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human CPT1A. The exact sequence is proprietary.

Reactivity Notes

Rat (82%), Dog (86%), Pig (89%), Sheep (85%), Bovine (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CPT1A Antibody

Western Blot: CPT1A Antibody [NBP2-15993]

Western Blot: CPT1A Antibody [NBP2-15993]

Western Blot: CPT1A Antibody [NBP2-15993] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [C1C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: CPT1A Antibody [NBP2-15993]

Immunohistochemistry-Paraffin: CPT1A Antibody [NBP2-15993]

Immunohistochemistry-Paraffin: CPT1A Antibody [NBP2-15993] - Mouse kidney. CPT1A stained by CPT1A antibody [C1C2], Internal diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: CPT1A Antibody [NBP2-15993]

Immunohistochemistry-Paraffin: CPT1A Antibody [NBP2-15993]

Immunohistochemistry-Paraffin: CPT1A Antibody [NBP2-15993] - MDA-MB157 xenograft, using CPT1A antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for CPT1A Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CPT1A

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Carnitine O-palmitoyltransferase 1, liver isoform

Alternate Names

CPT I, CPT1, CPT1-L, CPTI-L, EC 2.3.1.21

Gene Symbol

CPT1A

UniProt

P50416 (Human)

Additional CPT1A Products

Product Documents for CPT1A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CPT1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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