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CRABP2 Antibody (OTI10D6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70448

Novus Biologicals, part of Bio-Techne
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NBP2-70448

Key Product Details

Species Reactivity

Human, Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI10D6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human CRABP2 (NP_001869) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

15.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CRABP2 Antibody (OTI10D6) - Azide and BSA Free

Western Blot: CRABP2 Antibody (OTI10D6)Azide and BSA Free [NBP2-70448]

Western Blot: CRABP2 Antibody (OTI10D6)Azide and BSA Free [NBP2-70448]

Western Blot: CRABP2 Antibody (OTI10D6) - Azide and BSA Free [NBP2-70448] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRABP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRABP2.
Immunohistochemistry: CRABP2 Antibody (OTI10D6) - Azide and BSA Free [NBP2-70448]

Immunohistochemistry: CRABP2 Antibody (OTI10D6) - Azide and BSA Free [NBP2-70448]

Immunohistochemistry: CRABP2 Antibody (OTI10D6) - Azide and BSA Free [NBP2-70448] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CRABP2 mouse monoclonal antibody.
Flow Cytometry: CRABP2 Antibody (OTI10D6) - Azide and BSA Free [NBP2-70448]

Flow Cytometry: CRABP2 Antibody (OTI10D6) - Azide and BSA Free [NBP2-70448]

Flow Cytometry: CRABP2 Antibody (OTI10D6) - Azide and BSA Free [NBP2-70448] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-CRABP2 antibody, and then analyzed by flow cytometry.

Applications for CRABP2 Antibody (OTI10D6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CRABP2

Cellular retinoic acid binding protein 2 (CRABP2) is a coactivator present in the retinoic acid (RA)-dependent nuclear complex. CRABP2 has been shown to mediate retinoic acid activity in skin to influence skin cell growth and differentiation. It has also been shown to mediate the growth suppressive effects of retinoic acid in breast cancer. CRABP2's role in cell differentiation may be linked to control of the cell-cycle since it has been identified as an interacting partner of cyclin D3 in CRABPII and RAR ternary complex. Alternate names for CRABP2 include cellular retinoic acid-binding protein II, CRABP-II, retinoic acid-binding protein II, and RBP6.

Long Name

Cellular Retinoic Acid Binding Protein 2

Alternate Names

CRABP-II, RBP6

Gene Symbol

CRABP2

Additional CRABP2 Products

Product Documents for CRABP2 Antibody (OTI10D6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CRABP2 Antibody (OTI10D6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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