CRABP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99740

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CRABP2 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human CRABP2 (Accession#: NP_001869.1; Pro2-Glu 138)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CRABP2 Antibody

Western Blot: CRABP2 Antibody [NBP2-99740]

Western Blot: CRABP2 Antibody [NBP2-99740]

Western Blot: CRABP2 Antibody [NBP2-99740] - Anti-CRABP2 rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 14 kDa
Western Blot: CRABP2 Antibody [NBP2-99740]

Western Blot: CRABP2 Antibody [NBP2-99740]

Western Blot: CRABP2 Antibody [NBP2-99740] - CRABP2 was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate 2 ul anti-CRABP2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CRABP2 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 16 kDa

Applications for CRABP2 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

0.5-2 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRABP2

Cellular retinoic acid binding protein 2 (CRABP2) is a coactivator present in the retinoic acid (RA)-dependent nuclear complex. CRABP2 has been shown to mediate retinoic acid activity in skin to influence skin cell growth and differentiation. It has also been shown to mediate the growth suppressive effects of retinoic acid in breast cancer. CRABP2's role in cell differentiation may be linked to control of the cell-cycle since it has been identified as an interacting partner of cyclin D3 in CRABPII and RAR ternary complex. Alternate names for CRABP2 include cellular retinoic acid-binding protein II, CRABP-II, retinoic acid-binding protein II, and RBP6.

Long Name

Cellular Retinoic Acid Binding Protein 2

Alternate Names

CRABP-II, RBP6

Gene Symbol

CRABP2

Additional CRABP2 Products

Product Documents for CRABP2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CRABP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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