Cre Antibody (10212)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42832

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Cre/lox System

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 10212

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cre Antibodies »

Product Specifications

Immunogen

Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of Cre. The exact sequence is proprietary.

Reactivity Notes

Specific to Cre/lox system (Bacteriophage). Shown to detect Cre protein in Cre-transfected human cells (HeLa, 293T).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cre Antibody (10212)

Western Blot: Cre Antibody (10212) [NBP2-42832]

Western Blot: Cre Antibody (10212) [NBP2-42832]

Western Blot: Cre Antibody (10212) [NBP2-42832] - Analysis of A. IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins 12.5 % SDS-PAGE Cre recombinase antibody [10212] dilution: 1:10000
Western Blot: Cre Antibody (10212) [NBP2-42832]

Western Blot: Cre Antibody (10212) [NBP2-42832]

Western Blot: Cre Antibody (10212) [NBP2-42832] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug whole cell lysate/extract of Cre-tagged protein expressing HeLa cells 7.5 % SDS-PAGE Cre recombinase antibody [10212] dilution: 1:5000
Immunoprecipitation: Cre Antibody (10212) [NBP2-42832]

Immunoprecipitation: Cre Antibody (10212) [NBP2-42832]

Immunoprecipitation: Cre Antibody (10212) [NBP2-42832] - Analysis of 293T whole cell lysate/extract A : 20 ug whole cell lysate/extract of Cre recombinase protein expressing 293T cells B : Control with 2.5 ug of pre-immune mouse IgG C : Immunoprecipitation of Cre recombinase protein by 2.6 ug of Cre recombinase antibody 7.5% SDS-PAGE The immunoprecipitated Cre recombinase protein was detected by Cre recombinase antibody diluted at 1 : 5000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.

Applications for Cre Antibody (10212)

Application
Recommended Usage

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol (pH7)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cre

The Cre (cyclization recombination) enzyme, a member of a large family of recombinases, recognizes loxP which is a sequence motif of 34 bp from the PI bateriophage. If a DNA seqment is flanked by two loxP sites in the same orientation, Cre excises that seqment leaving a single loxP site in the remaining DNA molecule. The excised DNA segment is subsequently degraded. Cre and lox comprise the Cre/lox system which was first developed in the late 1980's to artificially manipulate gene expression (reviewed in Branda and Dymecki, 2004; Ghosh and Duyne, 2002; and Gilbertson, 2003). As an example, mice with the Cre protein expressing in a specific cell type are bred with mice that contain a target gene surrounded by loxP sites. When the mice are bred, the cells carrying Cre will cause those cells to lose the target gene. If the Cre gene is, for example, bound to a promoter that only allows Cre production in neuronal cells, the target gene will be deleted only in those cells. Since 1989 the Cre/lox system has been used extensively and there are numerous animal, plant and bacterial stocks that already contain the Cre gene driven by ubiquitous, tissue-specific or inducible promoters and provide a quick method for breeding experiments. The Cre/lox system has the advantage of working in almost any type of cell. This has led to its application in many experiments, for example in selectively labeling neuronal cells in the brain thereby differentiating them from other types of surrounding cells. This Cre antibody recognizes the recombinase protein, Cre. Cre is a 343 amino acid protein, GenBank no. AAQ14086.1.

Alternate Names

Cyclization recombinase

Additional Cre Products

Product Documents for Cre Antibody (10212)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cre Antibody (10212)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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