Key Product Details

Species Reactivity

Validated:

Mouse, Cre/lox System

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Cre Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a portion of bacteriophage protein CRE (within amino acids 50-100). [UniProt# P06956]

Reactivity Notes

Specific to Cre/lox system (Bacteriophage). Shown to detect Cre protein in Cre-transfected human cells (HeLa) and Cre-transgenic mice. Mouse reactivity reported in scientific literature (PMID: 28194015).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cre Antibody - BSA Free

Western Blot: Cre AntibodyBSA Free [NB100-56133]

Western Blot: Cre AntibodyBSA Free [NB100-56133]

Western Blot: Cre Antibody [NB100-56133] - Analysis of Cre using NB100-56133 at 1:2000. Lane 1: HeLa cells stably transfected with a CRE expression plasmid. Lane 2: HeLa cells transfected with a neo plasmid (negative control).
Immunohistochemistry: Cre Antibody - BSA Free [NB100-56133]

Immunohistochemistry: Cre Antibody - BSA Free [NB100-56133]

Immunohistochemistry: Cre Antibody - BSA Free [NB100-56133] - Representative co-IF image showing YFP (green) and Cre (red) staining on a MG section from a Trp53L/L;R26Y female 3 days after Ad-K8-Cre injection. Trp53L/L;R26Y females (n=3); R26Y females (n=3). Scale bar, 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28194015/) licensed under a CC-BY license.
Immunohistochemistry: Cre Antibody - BSA Free [NB100-56133]

Immunohistochemistry: Cre Antibody - BSA Free [NB100-56133]

Immunohistochemistry: Cre Antibody [NB100-56133] - FFPE tissue section from the cerebellum of a Cre transgenic mouse using NB100-56133 at 1:2000. In this mouse line, Cre is expressed in the brain, predominantly in the nuclei of most types of neurons. The arrow denotes an example of a stained neuron. Cre staining is also seen in the cytosol of cerebellar Purkinje cells. Hematoxylin-eosin counterstain.
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Applications for Cre Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:500

Immunohistochemistry-Frozen

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

1:50 - 1:200

Western Blot

1:1000 - 1:4000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB100-56133 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cre

Cre recombinase or cyclization recombination enzyme is a bacteriophage-P1 enzyme belonging to large family of recombinases which are required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state. Cre is well known for facilitating a site-specific recombination between two 34 base pair loxP sites. If a DNA seqment is flanked by two loxP sites in the same orientation, Cre excises that seqment leaving a single loxP site in the remaining DNA molecule, and the excised DNA segment is subsequently degraded. Cre and lox comprises Cre/lox system which has been used extensively for multiple in vitro and in vivo applications including targeted gene deletions, gene-specific humanized animal models, as well as in plants and microorganisms stocks.

Alternate Names

Cyclization recombinase

Additional Cre Products

Product Documents for Cre Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cre Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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