Creatine Kinase BB Antibody (1G10U9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16510
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1G10U9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Creatine Kinase BB Antibody (1G10U9)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Creatine Kinase BB (NP_001814.2). MPFSNSHNALKLRFPAEDEFPDLSAHNNHMAKVLTPELYAELRAKSTPSGFTLDDVIQTGVDNPGHPYIMTVGCVAGDEESYEVFKDLFDPIIEDRHGGY
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Creatine Kinase BB Antibody (1G10U9)
Western Blot: Creatine Kinase BB Antibody (1G10U9) [NBP3-16510]
Western Blot: Creatine Kinase BB Antibody (1G10U9) [NBP3-16510] - Western blot analysis of extracts of various cell lines, using Creatine Kinase BB Rabbit mAb (NBP3-16510) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.Immunohistochemistry-Paraffin: Creatine Kinase BB Antibody (1G10U9) [NBP3-16510]
Immunohistochemistry-Paraffin: Creatine Kinase BB Antibody (1G10U9) [NBP3-16510] - Immunohistochemistry of paraffin-embedded human appendix using Creatine Kinase BB Rabbit mAb (NBP3-16510) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Immunohistochemistry-Paraffin: Creatine Kinase BB Antibody (1G10U9) [NBP3-16510]
Immunohistochemistry-Paraffin: Creatine Kinase BB Antibody (1G10U9) [NBP3-16510] - Immunohistochemistry of paraffin-embedded rat heart using Creatine Kinase BB Rabbit mAb (NBP3-16510) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for Creatine Kinase BB Antibody (1G10U9)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Creatine Kinase BB
Alternate Names
B-CK, CKB
Gene Symbol
CKB
Additional Creatine Kinase BB Products
Product Documents for Creatine Kinase BB Antibody (1G10U9)
Product Specific Notices for Creatine Kinase BB Antibody (1G10U9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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