Creatine Kinase BB Antibody (CK-BYK/21E10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11251

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CK-BYK/21E10

Concentration

0.1 mg/ml

View all Creatine Kinase BB Antibodies »

Product Summary for Creatine Kinase BB Antibody (CK-BYK/21E10)

Immunogen

Synthetic 17-mer peptide corresponding to the N-terminal sequence of human creatine kinase brain-type (SNSHNALKLRFPAEDEF)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Creatine Kinase BB Antibody (CK-BYK/21E10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Creatine Kinase BB

The monoclonal antibody CK-BYK/21E10 recognizes human creatine kinase B-type, also known as B-CK. Human B-CK is a protein of 381 amino acids (~45 kDa), expressed in a number of tissues. B-CK is most abundant in adult brain, approx. 5-fold lower in the stomach, 10-fold lower in the heart and barely detectable in liver. In brain, whereas most B-CK has been shown to be cytosolic, several of the reactions requiring B-CK are membrane-associated. B-CK belongs to the creatine kinase (CK) isoenzymes that catalyse the synthesis of phosphocreatine (PCr) and its subsequent use in the regeneration of ATP in cell types where the consumption of ATP is rapid and/or sudden. In the brain the different CK isoforms constitute an energy shuttle wherein ATP produced in the mitochondria is used by a mitochondrial CK [e.g. ubiquitous mitochondrial creatine kinase (uMi-CK)] to generate PCr, which is then transported and used by a cytoplasmic CK [e.g. brain creatine kinase (B-CK)] to regenerate ATP at discrete cellular sites of high ATP turnover. B-CK appears to have a role in regenerating ATP needed for the transport of ions and neurotransmitters since CKB has been localized to brain synaptic plasma membranes, possibly coupled to Na+/K+-ATPase and acetylcholine receptor-rich membranes. Expression of B-CK is developmentally controlled: in rat, brain CK protein at birth is extremely low and increases 10-fold until week 4. This reflects the many energy-demanding processes in brain during brain development.

Alternate Names

B-CK, CKB

Gene Symbol

CKB

Additional Creatine Kinase BB Products

Product Documents for Creatine Kinase BB Antibody (CK-BYK/21E10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Creatine Kinase BB Antibody (CK-BYK/21E10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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