Creatine Kinase BB Antibody (CKBB/6565)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20318
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # CKBB/6565
Concentration
0.2 mg/ml
Product Summary for Creatine Kinase BB Antibody (CKBB/6565)
Immunogen
Recombinant human full-length Creatine Kinase BB protein
Localization
Cytoplasm.
Specificity
This monoclonal antibody recognizes the Creatine Kinase BBB isoenzyme and does not react with the B subunit in CKMB
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Positive Controls: SH-SY5Y or HEK-293 cells. Human cerebellum.
Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Non-hazardous. No MSDS required.
Scientific Data Images for Creatine Kinase BB Antibody (CKBB/6565)
Western blot analysis of Y79 cell lysate using Creatine Kinase BB antibody (CKBB/6565).
Creatine Kinase BB Antibody (CKBB/6565)
Formalin-fixed, paraffin-embedded human brain stained with Creatine Kinase BB antibody (CKBB/6565).
Creatine Kinase BB Antibody (CKBB/6565)
Formalin-fixed, paraffin-embedded human kidney stained with Creatine Kinase BB antibody (CKBB/6565).Applications for Creatine Kinase BB Antibody (CKBB/6565)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Creatine Kinase BB
Alternate Names
B-CK, CKB
Gene Symbol
CKB
Additional Creatine Kinase BB Products
Product Documents for Creatine Kinase BB Antibody (CKBB/6565)
Product Specific Notices for Creatine Kinase BB Antibody (CKBB/6565)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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