Creatine Kinase BB Antibody (CPTC-CKB-2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07542

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # CPTC-CKB-2

Concentration

0.2 mg/ml

View all Creatine Kinase BB Antibodies »

Product Specifications

Immunogen

Recombinant human full-length Creatine Kinase BB protein (Uniprot: P12277)

Localization

Cytoplasm

Specificity

Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This monoclonal antibody recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08866)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Creatine Kinase BB Antibody (CPTC-CKB-2)

Western Blot: Creatine Kinase BB Antibody (CPTC-CKB-2) [NBP3-07542]

Western Blot: Creatine Kinase BB Antibody (CPTC-CKB-2) [NBP3-07542]

Western Blot: Creatine Kinase BB Antibody (CPTC-CKB-2) [NBP3-07542] - Western Blot Analysis of HEK293 cell lysate using Creatine Kinase BB Mouse Monoclonal Antibody (CPTC-CKB-2).

Applications for Creatine Kinase BB Antibody (CPTC-CKB-2)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Product Documents for Creatine Kinase BB Antibody (CPTC-CKB-2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Creatine Kinase BB Antibody (CPTC-CKB-2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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