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Creatine kinase MT 1A Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05671

Novus Biologicals, part of Bio-Techne
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NBP3-05671-100ul
NBP3-05671-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 258-417 of human Creatine kinase MT 1A (NP_001015001.1). FLIWVNEEDHTRVISMEKGGNMKRVFERFCRGLKEVERLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHIKLPLLSKDSRFPKILENLRLQKRGTGGVDTAATGGVFDISNLDRLGKSEVELVQLVIDGVNYLIDCERRLERGQDIRIPTPVIHTKH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Creatine kinase MT 1A Antibody - BSA Free

Western Blot: Creatine kinase MT 1A AntibodyBSA Free [NBP3-05671]

Western Blot: Creatine kinase MT 1A AntibodyBSA Free [NBP3-05671]

Western Blot: Creatine kinase MT 1A Antibody [NBP3-05671] - Western blot analysis of extracts of various cell lines, using Creatine kinase MT 1A Antibody (NBP3-05671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Applications for Creatine kinase MT 1A Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Creatine kinase MT 1A

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to thecytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes,sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two differentoligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes.Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; thismay be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrialcreatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes locatednear each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins.(provided by RefSeq)

Alternate Names

Acidic-Type Mitochondrial Creatine Kinase, CKMT, CKMT1, CKMT1A, Creatine Kinase U-Type, Mitochondrial, Creatine Kinase, Mitochondrial 1 (Ubiquitous), Creatine Kinase, Mitochondrial 1A, EC 2.7.3.2, Mia-CK, Ubiquitous Mitochondrial Creatine Kinase, U-MtCK

Gene Symbol

CKMT1A

Additional Creatine kinase MT 1A Products

Product Documents for Creatine kinase MT 1A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Creatine kinase MT 1A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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