CRIPTO Antibody (JE48-67)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32220
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE48-67
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human CRIPTO aa 31-169. (Uniprot: P13385)
Localization
Cell membrane, Secreted.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CRIPTO Antibody (JE48-67)
Western Blot: CRIPTO Antibody (JE48-67) [NBP3-32220]
Western Blot: CRIPTO Antibody (JE48-67) [NBP3-32220] - Western blot analysis of CRIPTO on NCCIT cell lysates with Rabbit anti-CRIPTO antibody (NBP3-32220) at 1/1,000 dilution.Lysates/proteins at 20 ug/Lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32220) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for CRIPTO Antibody (JE48-67)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Cripto
CRIPTO-1 antibodies are useful tools for studying embryonic development and certian cancers.
Long Name
Teratocarcinoma-derived Growth Factor/Cripto Growth Factor
Alternate Names
CFC-2, TDGF-1, TDGF1
Gene Symbol
CRIPTO
Additional Cripto Products
Product Documents for CRIPTO Antibody (JE48-67)
Product Specific Notices for CRIPTO Antibody (JE48-67)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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