CRISP-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52013

Novus Biologicals, part of Bio-Techne
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NBP1-52013
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Key Product Details

Species Reactivity

Human, Porcine, Bovine, Canine

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all CRISP-2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence KCTLQHSDPEDRK corresponding to internal region according to NP_003287.1.

Epitope

KCTLQHSDPEDRK

Specificity

Reported variants represent identical protein: NP_003287.1, NP_001135879.1, NP_001135889.1, NP_001135907.1, NP_001135880.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CRISP-2 Antibody

Western Blot: CRISP-2 Antibody [NBP1-52013]

Western Blot: CRISP-2 Antibody [NBP1-52013]

Western Blot: CRISP-2 Antibody [NBP1-52013] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag probed with EB09850 (0.11ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB09850 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Applications for CRISP-2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.05 - 0.1 ug/ml
Application Notes
WB: In transfected HEK293 transiently expressing human CRISP2 (MYC and DYKDDDDK tagged), a band of approx. 35 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CRISP-2

CRISP2, or Cysteine-rich secretory protein 2, consists of a 243 amino acid isoform that is 27 kDa, and is involved in the regulation of calcium fluxation and ion channel activity during sperm capacitation. Current research is being conducted on CRISP2 and its relation to several diseases and disorders, including male infertility, atopic dermatitis, and sinusitis. The protein is not currently linked to any specific pathways or biological processes, but it interacts with PPP2R1A and PPP2R1B.

Long Name

Cysteine-rich Secretory Protein 2

Alternate Names

CRISP2, GAPDL5, TPX1, TSP1

Entrez Gene IDs

7180 (Human)

Gene Symbol

CRISP2

UniProt

NP_003287.1 (Human)

Additional CRISP-2 Products

Product Documents for CRISP-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CRISP-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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