Crk [p Tyr251] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23255
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken, Xenopus
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Crk [p Tyr251] Antibody
Immunogen
Phospho-Crk (Tyr-251) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-251 in human Crk II. This peptide sequence is well conserved in mouse and rat Crk II, as well as in CrkL (Tyr-251). The site is not found in Crk I. [UniProt# P46108]
Modification
p Tyr251
Specificity
The antibody detects a 38 and 40 kDa* proteins corresponding to the molecular mass of Crk II and CrkL on SDS-PAGE immunoblots of human K562 cells stimulated with pervanadate and A431 cells stimulated with EGF. This reactivity is not observed after akaline phosphatase treatment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Crk [p Tyr251] Antibody
![Crk [p Tyr251] Antibody](https://resources.bio-techne.com/images/products/nbp3-23255_rabbit-crk-p-tyr251-pab-19920231252366.jpg "Western Blot: Crk [p Tyr251] Antibody [NBP3-23255] -")
Western Blot: Crk [p Tyr251] Antibody [NBP3-23255] -
Western Blot: Crk [p Tyr251] Antibody [NBP3-23255] - Western blot of human K562 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1-6). The blot was treated with akaline phosphatase to dephosphorylate Crk (lanes 2, 4 & 6), then the blot was probed with rabbit polyclonals CrkL (C-terminus) NBP3-23256 (lanes 1 & 2), Crk (Tyr-251) phospho-specific NBP3-23255 (lanes 3 & 4), and mouse monoclonal anti-Crk (C-terminal region) (lanes 5 & 6).Applications for Crk [p Tyr251] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Crk
Long Name
V-Crk Sarcoma Virus CT10 Oncogene Homolog
Alternate Names
adapter molecule crk, avian sarcoma virus CT10 (v-crk) oncogene homolog, CRKII, p38, Proto-oncogene c-Crk, v-crk avian sarcoma virus CT10 oncogene homolog, v-crk sarcoma virus CT10 oncogene homolog (avian)
Gene Symbol
CRK
Additional Crk Products
Product Documents for Crk [p Tyr251] Antibody
Product Specific Notices for Crk [p Tyr251] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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