Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot (Negative)

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CRM1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 375 and 425 of human Chromosome Region Maintenance 1 (Exportin 1) using the numbering given in entry NP_003391.1 (GeneID 7514).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CRM1 Antibody

Immunoprecipitation: CRM1 Antibody [NB100-79811]

Immunoprecipitation: CRM1 Antibody [NB100-79811]

Immunoprecipitation: CRM1 Antibody [NB100-79811] - Detection of CRM1 (Exportin 1) by Immunoprecipitation. Samples: Whole cell lysate (1 mg/IP; 1/4 of reaction loaded/lane) from HeLa cells. Antibodies: Affinity purified rabbit anti-CRM1 antibody BL1956 (Cat. No. NB100-79811) used at 3 mcg/mg lysate for IP.

Applications for CRM1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CRM1

CRM1 mediates the nuclear export of cellular proteins (cargoes) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, CRM1 binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The direction of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. CRM1 also mediates the nuclear export of the Rev protein of HIV-1 and Rex protein of HTLV-1. It is involved in Rex dimerization, U3 snoRNA transport from Cajal bodies to nucleoli. CRM1 is also involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. CRM1 is also involved in the regulation NFAT and AP-1. Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in an approx. 25 nm channel used for larger, actively transported molecules. Active transport of molecules through the NPC is a signal-mediated process that is not well understood. Proteins to be exported from the nucleus to cytoplasm contain leucine-rich nuclear export signals (NESs). NESs are recognized by transport receptors belonging to the importin-beta family of proteins. It has been shown that NES export is mediated by CRM 1 (Chromosome Region Maintenance 1), an importin-beta family member that binds NESs, Ran:GTP, and Ran binding protein (RanBP). CRM 1 mediates the nuclear exportation of numerous proteins, including histone deacetylases (HDACs), viral proteins, immunophilins, and STAT 1.

Long Name

Chromosome Region Maintenence 1 Homolog

Alternate Names

CRM-1, Exp1, Exportin 1, XPO1

Entrez Gene IDs

7514 (Human)

Gene Symbol

XPO1

UniProt

NP_003391.1 (Human)

Additional CRM1 Products

Product Documents for CRM1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CRM1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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