CRMP4 Antibody (JE55-99)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32225
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE55-99
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human CRMP4 aa 451-500 / 570. (Uniprot: Q14195)
Localization
Cytoplasm. Cell projection.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CRMP4 Antibody (JE55-99)
Western Blot: CRMP4 Antibody (JE55-99) [NBP3-32225]
Western Blot: CRMP4 Antibody (JE55-99) [NBP3-32225] - Western blot analysis of CRMP4 on different lysates with Rabbit anti-CRMP4 antibody (NBP3-32225) at 1/1,000 dilution.Lane 1: SH-SY5Y cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Human brain tissue lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Rat brain tissue lysate
Cell lysates/proteins at 20 ug/Lane.
Tissue lysates/proteins at 30 ug/Lane.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32225) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for CRMP4 Antibody (JE55-99)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CRMP4
Alternate Names
Collapsin response mediator protein 4, CRMP-4, CRMP4collapsin response mediator protein 4 long, dihydropyrimidinase-like 3, DRP-3dihydropyrimidinase-related protein 3, DRP3ULIP1, ULIP-1, ULIPLCRMP, Unc-33-like phosphoprotein 1
Gene Symbol
DPYSL3
Additional CRMP4 Products
Product Documents for CRMP4 Antibody (JE55-99)
Product Specific Notices for CRMP4 Antibody (JE55-99)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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