Crotonaldehyde Antibody (2D3.1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61922
Conjugate
Catalog #
Key Product Details
Species Reactivity
Non-species specific
Applications
Dot Blot, ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2D3.1
Concentration
1 mg/ml
Product Specifications
Immunogen
Crotonaldehyde modified Keyhole Limpet Hemocyanin (KLH)
Reactivity Notes
Species Independent
Specificity
Specific for Crotonaldehyde modified proteins. Does not cross-react with Acrolein, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Crotonaldehyde Antibody (2D3.1)
Dot Blot: Crotonaldehyde Antibody (2D3.1) [NBP2-61922] - Dot blot analysis using Mouse Anti-Crotonaldehyde Monoclonal Antibody, Clone 2D3.1 (NBP2-61922). Primary Antibody: Mouse Anti-Crotonaldehyde Monoclonal Antibody (NBP2-61922) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.
Applications for Crotonaldehyde Antibody (2D3.1)
Application
Recommended Usage
ELISA
1:1000
Western Blot
1:1000
Application Notes
A 1:1000 dilution of this antibody was sufficient for detection of Crotonaldehyde-modified proteins in 1 ug of Crotonaldehyde-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Crotonaldehyde
Alternate Names
CRA;CRA-lysine adduct;Croton aldehyde;Crotonaldehyde
Additional Crotonaldehyde Products
Product Documents for Crotonaldehyde Antibody (2D3.1)
Product Specific Notices for Crotonaldehyde Antibody (2D3.1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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