Crry Antibody (8A/E6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11264

Novus Biologicals, part of Bio-Techne
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NBP3-11264
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Key Product Details

Species Reactivity

Mouse

Applications

Immunoassay, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 8A/E6

Concentration

0.1 mg/ml

View all Crry Antibodies »

Product Specifications

Immunogen

Mouse Crry fused to mouse IgG2a-Fc

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Crry Antibody (8A/E6)

Western Blot: Crry Antibody (8A/E6) [NBP3-11264]

Western Blot: Crry Antibody (8A/E6) [NBP3-11264]

Western Blot: Crry Antibody (8A/E6) [NBP3-11264] - Analysis of HEK293T cells transfected with AAV9 plasmid carrying sCrry.

Applications for Crry Antibody (8A/E6)

Application
Recommended Usage

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Crry

The monoclonal antibody 8A/E6 recognizes mouse complement receptor type-1 related gene Y (Crry/p65), a type I membrane protein (MW 56-60 kDa). Complement comprises a system of soluble serum proteins that, upon activation by antibody (classical pathway), sugars (lectin pathway), or bacterial and foreign surfaces (alternative pathway), directly causes cell damage. Complement is constantly activated at low levels in serum, requiring cells to protect themselves from damage. In human, cells are protected from complement-mediated damage by expression of membrane-bound complement regulatory proteins, which act at serveral points along the cascade. Human cells are protected by decay-accelerating factor (DAF, CD55), membrane cofactor protein (MCP, CD46) and membrane attack complex inhibitor protein 1 (MIN1, CD59). Rat and mouse express a unique membrane complement regulator of the activating pathways, called complement receptor type-1 related gene Y (Crry). Crry/p65 is expressed on a wide variety of cells. Crry/p65 contains tandem short consensus repeats (SCR) characteristic of C3/C4 binding proteins. Mouse Crry/p65 has been shown to regulate both classical and alternative complement pathway C3 deposition on cell membranes. Crry/p65 must, therefore, exert its effects prior to, or at the level of, the C3 convertases, in a fashion similar to that of the human membrane factors DAF and MCP. Crry/p65 is an evolutionarily unique, complement regulatory protein which has developed in mouse and rat. Besides the involvement in control of complement damage to cell membranes Crry has also a role in T cell activation. Crry basically enhances the signals triggered by TCR/CD3 activation.

Alternate Names

Complement component (3b/4b) receptor 1-like, Complement component receptor 1-like protein, Complement receptor type-1 related gene Y, Complement regulatory protein Crry, CR, Cr1l, CRY, MCP, mCR, mCRY, p65, Protein p65

Gene Symbol

Cr1l

Additional Crry Products

Product Documents for Crry Antibody (8A/E6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Crry Antibody (8A/E6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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