CRTAP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-94105
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LVYYQYHRDTWGLSDEHFQPRPEAVQFFNVTTLQKELYDFAKENIMDDDEGEVVEYVDDLLELEET
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CRTAP Antibody
Western Blot: CRTAP Antibody [NBP1-94105]
Western Blot: CRTAP Antibody [NBP1-94105] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: CRTAP Antibody [NBP1-94105]
Immunocytochemistry/Immunofluorescence: CRTAP Antibody [NBP1-94105] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & actin filaments.Immunohistochemistry-Paraffin: CRTAP Antibody [NBP1-94105]
Immunohistochemistry-Paraffin: CRTAP Antibody [NBP1-94105] - Staining of human kidney shows strong cytoplasmic positivity in cells in renal tubules.Applications for CRTAP Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CRTAP
Alternate Names
cartilage associated protein, cartilage-associated protein, CASPLEPREL3, leprecan-like 3
Gene Symbol
CRTAP
Additional CRTAP Products
Product Documents for CRTAP Antibody
Product Specific Notices for CRTAP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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